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Capture Compounds towards a targeted reduction of proteome complexity
Erik Duelsner, Aysel Alici, Christian Jurinke, Hubert Koester. caprotec bioanalytics GmbH

Capture Compound Mass Spectrometry (CCMS) enables the enrichment of proteins based on their functionality. CCMS is commercialized as kits for research applications and in collaborations with pharmaceutical companies. The focus is on investigating mechanisms of drug action and avoidance of toxic effects of small molecule drugs.

A Novel Approach to Internal Standardization in LC/MS/MS Analysis; Sensitive LC/MS/MS Analysis of Gentamicin
Bruce Babson, Noel Henderson and Nicholas Chestara. MicroConstants, Inc., 9050 Camino Santa Fe, San Diego, CA 92121

A novel approach to internal standardization in LC/MS/MS is demonstrated. Gentamicin, a multi-component aminoglycoside, is derivatized with propyl-d7 chloroformate making the internal standard. Gentamicin components in the samples are derivatized with non-labeled propyl chloroformate making them analogous to the internal standard. Additional benefits of the derivatization are increased hydrophobicity and excellent MS/MS fragmentation allowing for a sensitive LLOQ of 2 ng/mL total Gentamicin usin

A novel methode for coupling capillary columns
Wil van Egmond, Daniela Peroni, . NLISIS, University of Amsterdam

Using a proprietary, low-melting, deactivated glass we have developed a novel instant Meltfit™ connection. Reliable connectors are made in-situ in less than a minute.

Single stage reversed-phase liquid chromatography-mass spectrometry for the characterization of triglyceride positional isomers.
Haslina Hasan, Brendan J Keely and Julie Wilson. University Of York

The high level of selectivity afforded by RP-HPLC in combination with CID allowed recognition of eight groups of TAG positional isomers. Development of UPLC approach has reduced the run time by half while maintaining separation. The improved method combined with principal components analysis was used to assess the extent of variation of major TAGs in subcutaneous and intermuscular pork fat from four different locations of adipose tissues.

An improved data processing pipe-line for comprehensive H-NMR and X/MS -omics data
R. J. O. Torgrip, K. M. Åberg, E. Alm. Dept. Analytical Chemistry

In the post acqusition analysis of comprehensive -omics data the pre-processing pipe-line is crucial to extract the maximum possible amount of information from the data. Here we show a processing pipe-line for (1D)H-NMR and (2D)X/MS data comprising; feature detection, inter-sample feature alignment and internal-standard free normalization that outperforms today’s state-of-the-art processing schemes.

Five noncovalent peptidic ligands show different affinity rankings in solution and gas phase
Andrey Dyachenko , Michael Goldflam , Marta Vilaseca , Ernest Giralt . Institute for Research in Biomedicine (IRB), Barcelona Science Park, University of Barcelona, Spain

Stability of noncovalent complexes of VEGF protein with 5 peptidic ligands is studied. Experiments were conducted in solution (NMR CSP, ITC) and in gas phase (CID TOF MS). Each ligand differs from others in chirality of one amino acid. It was shown, that trend of stability of the studied noncovalent complexes is reversed in the gas phase relatively to the solution. An explanation of this behavior is presented.

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